《植物生理学报》 2015, 51(6): 921-934

番茄根特异基因的表达分析

冯婵莹^1,2,3, 李涛^2,3,*, 黎振兴², 李植良², 徐小万^2,3, 丁丽雪^1,2,3, 王永飞^1,*

¹暨南大学生命科学技术学院, 广州510532; ²广东省农业科学院蔬菜研究所, 广州510640; ³广东省蔬菜新技术研究重点实验室, 广州510640

通信作者：李涛；E-mail: tianxing84@163.com, msmwdw@163.com；Tel: 020-38469456, 020-32742408

摘　要：

根特异表达基因与根的生长发育及功能密切相关。本研究以根特异表达基因Solyc02g084780的表达模式为模板, 在番茄表达谱中筛选获得95个根特异表达基因; 在拟南芥和水稻中通过同源比对分别得到93和95个直系同源基因。对3个物种的根特异表达基因的表达模式开展研究, 结果表明直系同源基因的表达变异与进化关系相关。根特异表达基因的启动子分析结果表明, 基因的表达模式与启动子中调控元件的特性有关; 并对番茄8个根特异表达基因进行RT-PCR验证。

关键词：番茄; 根; 特异表达基因; 启动子分析

收稿：2015-01-18   修定：2015-05-04

资助：国家自然科学基金(31301776)、农业部现代产业体系项目(CARS-25-G-36)、农业科技成果转化资金项目(2013-GB2-E000361)、广东省对外科技合作项目(2013B050800012)、广东省省级科技计划项目(2014A020208044)、广东省科技厅条件建设项目(粤财教[2013]112号)和广东省农科院院长基金项目(201303)。

Expression Analysis of Root-Specific Genes in Tomato

FENG Chan-Ying^1,2,3, LI Tao^2,3,*, LI Zhen-Xing², LI Zhi-Liang², XU Xiao-Wan^2,3, DING Li-Xue^1,2,3, WANG Yong-Fei^1,*

¹Department of Biotechnology, Jinan University, Guangzhou 510632, China; ²Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; ³Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China

Corresponding author: LI Tao; E-mail: tianxing84@163.com, msmwdw@163.com; Tel: 020-38469456, 020-32742408

Abstract:

There is a close relation between root-specific gene expression and root development and functions. Using the expression pattern of Solyc02g084780 as a model, 95 root-specific genes had been found in tomato expression profile data. By homologous comparison, 93 and 95 orthologous homologous genes were found in Arabidopsis thaliana and rice, respectively. After comparing the expression pattern of orthologous homologous genes among these plants, the results showed that the expression pattern was different, and the expression pattern variation was similar in closer species while it’s greater in further species. The promoter analysis indicated that the difference of genes expression pattern was due to the characters of cis-elements in promoters, which was further proved by RT-PCR results of 8 randomly selected genes.

Key words: tomato (Solanum lycopersicum); root; specifically-expressed genes; promoter analysis